Fig. 2.

Genetic inhibition of sEH by gene KO decreases bone loss similar to chemical inhibitor. sEH^−/− and wild-type C57BL/6 mice were from a University of California, Davis–maintained colony. Mice at age 6 weeks were infected with A. actinomycetemcomitans three consecutive times, as described for Fig. 1, and at the end of the treatment period distance (µm) between the cement–enamel junction and the alveolar bone crest for all experimental groups was quantified. In parallel to the results with the sEH inhibitor TPPU, the genetic KO of sEH resulted in significantly reduced bone loss (Panel A). Panels display wild-type sham-infected (B, n = 8), sEH^−/− sham-infected (C, n = 15), wild-type mice orally infected with A. actinomycetemcomitans (D, n = 14), and sEH^−/− mice orally infected with A. actinomycetemcomitans (E, n = 14). The dark stained areas indicate sites of bone loss. The results are expressed as mean ± S.E.M. (*P < 0.001, one-way ANOVA followed by Student’s Newman–Keuls post hoc all pairwise comparison). wt, wild type.