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Initial velocity pattern showing a series of parallel lines characteristic of a ping-pong kinetic mechanism. (A) Glutamate as the variable substrate and fixed α-ketoisocaproate concentrations of 20 (●), 35 (■), 45 (▲), 100 (▼), and 300 μM (◆). (B) α-Ketoisocaproate as the variable substrate and fixed glutamate concentrations of 0.4 (●), 0.5 (■), 0.7 (▲), 1 (▼), and 2 mM (◆).
