Table 1.

Summary of MtIlvE Michaelis–Menten Constants in the Presence of Different α-Keto Acids and L-Glutamate^a

substrate	KM (×10−3 M)	V (s−1)	V/K (×104 M−1 s−1)
L-glutamate	1.30 ± 0.2	8.9 ± 0.4	0.7
α-keto-methyl-oxopentanoic acid	0.10 ± 0.01	6.2 ± 0.2	6.2
α-ketoisocaproate	0.24 ± 0.03	9.1 ± 0.4	3.8
α-ketoisovalerate	0.16 ± 0.01	5.9 ± 0.1	3.6
α-keto-phenylpyruvic acid	0.64 ± 0.1	1.4 ± 0.04	0.2
α-keto-γ-methylthiobutyric acid	6.07 ± 0.3	8.7 ± 0.1	0.2

^aV is the constant for the catalytic turnover of the enzyme. K is the substrate concentration necessary for the enzyme to reach half of its V_max. V/K is the substrate specificity.