Figure 4. Drug sensitivities of IGROV1 cells to AGF94 and PMX at leucovorin (LCV) concentrations of 2 and 25 nM.

Cells were plated (4000 cells/well) in folate-free RPMI 1640 medium with 10% dialyzed serum, antibiotics, L-glutamine, and 2 or 25 nM LCV with a range of concentrations of AGF94 or PMX. Cell proliferation was assayed with CellTiter-Blue™ and a fluorescent plate reader. Results for drug treatments were normalized to relative growth in the absence of drug additions. Results are shown as mean values +/− standard errors (error bars) from 6 separate experiments. IC₅₀s for AGF94 were 0.39 +/− 0.06 nM and 4.14 +/− 0.94 nM at 2 and 25 nM LCV, respectively. IC₅₀s for PMX were 57.7 +/− 3.0 nM and 346 +/− 26 nM at 2 and 25 nM LCV, respectively.