FIGURE 1.

Effects of hemolysis on plasma iron and zinc concentrations in vitro. The trace mineral concentration increased as a function of hemolysis as indicated by the hemoglobin concentration. (A) Plasma iron concentrations increased with the addition of hemolysate to nonhemolyzed plasma. Values are means ± SDs, n = 60. Data were fit to a linear function (solid line, R² = 0.918) with a 95% confidence band (dashed line) also shown. The inset shows data on a log₁₀ scale. (B) Plasma zinc concentrations increased with the addition of hemolysate to nonhemolyzed plasma at ∼1 g hemoglobin/L. Values are means ± SDs, n = 60. Data were fit to a linear function (solid line, R² = 0.698) with a 95% confidence band (dashed line) also shown. The inset shows data on a log₁₀ scale. (C) Iron and zinc concentrations were plotted for each plasma sample. Gray shading indicates the clinical reference range for plasma iron and zinc concentration as indicated (20). Values are means ± SDs, n = 60. Data were fit to a linear function (solid line, R² = 0.274).