Fig 5. Association of c-Src with CIB1-FAK complex augments its activity.

(A) Washed platelets were exposed to BSA or adhered to Fg for 45 min. The pre-cleared detergent lysates of these platelets were immunoprecipitated with anti-CIB1 or cIgG. Autoradiogram (AR) of MBP phosphorylation (upper panel). Western blot of immunoprecipitates probed with anti-Src (upper middle), and with anti-FAK (lower middle). The membrane was reprobed with anti-CIB1 to ensure equal amount of protein in the precipitates (lower panel). (B) Densitometric analysis of co-immunoprecipitated c-Src (upper middle blot in (A) (**P < 0.01). Error bars indicate mean ± SEM of at least three independent experiments. (C) Src kinase activity in CIB1 immunoprecipitates from platelets attached to Fg compared to BSA (***P<0.001).