Fig 2. Simplified metabolic network for MFA of Chlamydomonas reinhardtii.

The network consists of plastidic and cytosolic glycolysis and pentose phosphate pathway. The acetate imported (v₆₉) can be metabolized through acetyl CoA synthetase (v₁₇), cytosolic citrate synthase (v₇₀) and plastidic acetyl CoA synthetase/acetate kinase (v₆₈). Possible pathway for plastidic gluconeogenesis (-.-). Biomass fluxes are represented by (—). Metabolite abbreviations: G6P: glucose-6-phosphate, F6P: fructose-6-phosphate, T3P: Dihydroxyacetone phosphate and 3-phosphoglycerate, P5P: Pentose Phosphates: Ribulose-5-phosphate, Xylulose-5-phosphate, AcCoA: Acetyl-coA, PEP: phosphoenol pyruvate, Pyr: pyruvate, CIT: Citrate, ICT: isocitrate, AKG: alpha-ketoglutarate, SUC: succinate, MAL: Malate, OAA: oxaloacetate, GOX: glyoxylate, E4P: erythrose-4-phosphate, S7P:sedoheptulose-7-phosphate.