Fig 10. RRF/EF-G do not release polyU from polyU/ribosome/tRNA^phe complex.

(A) Lack of release of polyU from polyU/ribosome/tRNA^phe complex. The reaction mixture (1 ml) for the formation of the complex contained 8 mg of ribosome, 4.1 mg of tRNA^phe, 0.5 mg of polyU, 140 mM Tris-HCl pH 7.6, 60 mM NH₄Cl, 6 mM β-mercaptoethanol and 6 mM Mg(OAC)₂. When prepared in the presence of 6 mM Mg(OAC)₂, only one tRNA is bound to the P site of the ribosome [29]. The mRNA release was analyzed as described in the Materials and Methods section. (B) Time course of the release of tRNA^phe from polyU/ribosome/tRNA^phe complex prepared as described in (A). The tRNA release was measured as described in the Materials and methods.