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. 2017 May 24;12(5):e0177935. doi: 10.1371/journal.pone.0177935

Fig 3. Knockdown activity of the AgoshRNAs against luciferase-CCR5.

Fig 3

(A) Luciferase knockdown by the AgoshRNA was determined by co-transfection with the AgoshRNA constructs. HEK293T cells were co-transfected with 100 ng of the firefly luciferase reporter plasmid, 1 ng of renilla luciferase plasmid, and 25 ng of the corresponding AgoshRNA constructs. pBluescript (pBS) plasmid and an unrelated shRNA (shNef) served as negative control. The luciferase activity scored with shNef activity was set at 100%. (B) Luciferase knockdown by the most potent AgoshRNA constructs is dose-dependent when HEK293T cells were co-transfected with firefly luciferase reporter plasmid, renilla luciferase plasmid and increasing amount of AgoshRNA constructs (1, 5 and 25 ng). The mean values and standard deviation are based on six independent transfections.