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. 2017 May 24;12(5):e0178147. doi: 10.1371/journal.pone.0178147

Fig 7. Overexpression of TLR4 in low-glucose-treated RPTC suppresses wound healing.

Fig 7

RPTC were infected with lentiviruses containing TLR4 or control lentivirus and then subjected to low glucose and high glucose treatment for 2 days, followed by a scratch-wound healing assay. (A) Increased TLR4 levels were detected in the low-glucose- and high-glucose-treated RPTC. (B) MCP-1 mRNA level in low-glucose-treated and high-glucose-treated RPTC cells after TLR4 overexpression. (C) Overexpression of TLR4 in low-glucose-treated cells also suppressed wound healing, mimicking the effect of high glucose levels. (D) RPTC cells were infected with lentivirus containing TLR4 or control lentivirus, then cultured for 18 h in low glucose or high glucose medium with or without TAK-242, followed by a scratch-wound healing assay. Data are expressed as the mean ± S.D. (n = 4). *, p<0.05 versus the low glucose group; #, p<0.05 versus the group without TAK-242.