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. 2017 May 24;3(5):e1601898. doi: 10.1126/sciadv.1601898

Fig. 5. CTCF modulates the recruitment of BRCA2 to FokI-induced DSB.

Fig. 5

(A) U2OS-LacI-FokI-mCherry DSB reporter cells infected with control or CTCF shRNA were treated with Shield-1 and 4-hydroxytamoxifen for 6 hours to induce FokI expression as per Materials and Methods. Cells were next fixed and immunostained with the indicated antibodies and DAPI. (B) Quantification of colocalization between mCherry-FokI and 53BP1, LIGIV, BRCA2, γH2A.X, and Rad51. Error bars correspond to means ± SEM (n = 3; *P ≤ 0.05, two-tailed Student’s t test). (C) U2OS Ctl or CTCF knockdown cells were treated with NCS (150 ng/ml) for 3 hours followed by fixation and staining with the indicated antibodies. Representative images are shown. (D) Quantification of Ctl or CTCF knockdown cells harboring five or more 53BP1, BRCA1, or RAD51 foci. Error bars correspond to means ± SEM (n = 3; *P ≤ 0.05, paired Student’s t test).