Figure 3.

Cholestasis-induced hepatic dyslipidaemia is attenuated in TRPC5 KO mice. WT and TRPC5 KO mice were fed control (RM3) or CA (0.5% cholic acid supplemented RM3 diet) for 21 days, and (a) liver triglyceride (TG), total cholesterol (b), and LDL/VLDL (c) concentrations were measured. Hepatic gene expression determined by real-time quantitative PCR of genes involved in lipid biosynthesis (d), sterol regulatory element-binding transcription factor 1 (Srebf1), fatty acid synthase (Fasn), stearoyl-Coenzyme A desaturase 1 (Scd1) and (e) apolipoprotein E (Apoe), normalized to HPRT, β-actin, and B₂M. Results are mean ± S.E.M., n = 6–7. *P < 0.05, ***P < 0.001 vs control; ^#P < 0.05 vs WT CA as determined by two-way ANOVA and Bonferronni Post Hoc tests.