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. 2017 May 24;7:2332. doi: 10.1038/s41598-017-02216-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tie2 expression on mouse peripheral blood neutrophils. (A) Ly6G positivity was used to gate on neutrophils using forward and side scatter and Tie2 expression demonstrated as a log shift in fluorescence (FL-2) compared to isotype control. (B) For quantification of expression in unpermeabilised and permeabilised neutrophils, the mean fluorescence intensity (MFI) was determined. Results are presented as mean ± SEM (n = 3) and analysed for statistical significance using two-way ANOVA followed by Bonferroni’s post test for multiple comparisons. (C) Immunoprecipitation with anti-human Tie2 antibody resolved by SDS-PAGE- and probed with anti-mouse Tie2 antibody. Tie2 protein is detected at approximately 140 kDa. Lane 1: HUVEC lysate (positive control); lane 2: Mouse neutrophil immunoprecipitate.