Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 24;7:2316. doi: 10.1038/s41598-017-02583-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ET1 and its receptors (ETARs and ETBRs) are mainly present at glutamatergic neurons in the BLA. (a) Coronal sections of the BLA of C57 mice were stained with anti-CamKII antibody, anti-ET1 antibody and DAPI (blue). Most the right image shows combinations of red (CamKII), green (ET1) and blue (DAPI) channels. Unfilled triangles, ET1-positive neurons without CamKII; arrows, ET1- and CamKII-positive neurons. (b) Coronal sections of the BLA were stained with anti-GAD antibody, anti-ET1 antibody and DAPI. Most the right image shows combinations of red (GAD), green (ET1) and blue (DAPI) channels. Unfilled triangles, ET1-positive neurons without GAD; arrows, ET1- and GAD-positive neurons.(c) Quantitative analysis of pyramidal neurons and GABAergic neurons that are positive for ET1. (d) Coronal sections of the BLA were stained with anti-CamKII antibody, anti-ETAR antibody and DAPI. Most the right image shows combinations of red (CamKII), green (ETAR) and blue (DAPI) channels. Unfilled triangles, ETAR-positive neurons without CamKII; arrows, ETAR- and CamKII-positive neurons. (e) Coronal sections of the BLA were stained with anti-GAD antibody, anti-ETAR antibody and DAPI. Most the right image shows combinations of red (GAD), green (ET1) and blue (DAPI) channels. (f) Quantitative analysis of pyramidal neurons and GABAergic neurons that were positive for ETAR. Unfilled triangles, ETAR-positive neurons without GAD; arrows, ETAR- and GAD-positive neurons. (g) Coronal sections of the BLA were stained with anti-CamKII antibody, anti-ETBR antibody and DAPI. Most the right image shows combinations of red (CamKII), green (ETBR) and blue (DAPI) channels. Unfilled triangles, ETBR-positive neurons without CamKII; arrows, ETBR- and CamKII-positive neurons. (h) Coronal sections of the BLA were stained with anti-GAD antibody, anti-ETBR antibody and DAPI. Most the right image shows combinations of red (GAD), green (ETBR) and blue (DAPI) channels. Unfilled triangles, ETBR-positive neurons without GAD; arrows, ETBR- and GAD-positive neurons. (i) Quantitative analysis of pyramidal neurons and GABAergic neurons that were positive for ETBR. Scale Bar = 50 µm. Shown are means ± SE; n = 40 for CamKII-positive cells of 10 independent sections; n = 10 for GAD-positive cells of 10 independent sections. The 10 independent sections of each group were randomly selected from 5 mice. ***P < 0.001, Student’s t test for 3c, 3f, 3i.