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. 2017 May 24;7:2355. doi: 10.1038/s41598-017-02585-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of ANO1 in myocardial cell and cardiac fibroblasts by qRT-PCR (A), western bloting (B,C), and immunofluorescent confocal microscopic analysis (D). (A) qRT-PCR showed the relative mRNA level of Ano1 in myocardial cell and cardiac fibroblasts. (B,C) Western blotting demonstrated the expression of ANO1 protein in myocardial cell and cardiac fibroblasts. (D) Immunoflurescent confocal microscopy showed ANO1 protein expression profiles, cardiac fibroblasts were identified by vitemtin staining (red), ANO1 protein was green staining, and nuclei were identified by DAPI staining (blue). MC as myocardial cell, CF as cardiac fibroblasts. *p < 0.05, **p < 0.01.