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. 2017 May 24;7:2355. doi: 10.1038/s41598-017-02585-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of ANO1 reduced the indicators of cardiac fibrosis after hypoxia. (A) Ano1 mRNA was dramatically increased in ANO1 overexpression group comparing to negative control (NC) group by qRT-PCR. (B and C) ANO1 protein expression were dramatically increased in ANO1 overexpression group comparing to NC group by western blotting assay. (D) MTT analysis showed that cell viability elevated in overexpression ANO1 + hypoxia 16 hours (overexpression H) group comparing to overexpression ANO1 (over H) group and negative control (NC) + hypoxia 10 hours (NC H) group. (E and F) Expression of a-SMA protein was reduced in overexpression H group comparing to overexpression group and NC H group by western blotting. (G and H) Expression of collagen I protein was reduced in overexpression H group comparing to overexpression group and NC H group by western blotting. *p < 0.05, **p < 0.01.