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. 2017 May 24;7:2355. doi: 10.1038/s41598-017-02585-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of ANO1 attenuates cardiac fibrosis post-MI. (A)Western blot of ANO1 expression levels in Ad-ANO1+MI and Ad-GFP+MI group. The blots of GAPDH serves as loading controls. (B) Quantitative analysis of ANO1. (C) Representative Masson’s trichrome staining in sham group and remote zone, border zone, infarct zone of MI group. Blue staining indicates connective tissue. Scale bars represent 20 μm. (D) Representative Masson’s trichrome staining in sham, MI, Ad-ANO1+MI and Ad-GFP+MI. Scale bars represent 20 μm. (E) The quantification of fibrosis. *P < 0.05 versus MI or Ad-GFP+MI.**P < 0.01 versus sham. n = 6 per group.