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. 2017 May 24;7:2355. doi: 10.1038/s41598-017-02585-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ANO1 inhibited myofibroblast differentiation in vivo. (A) Western blot of a-SMA protein expression. GAPDH was used as loading controls. (B) Quantitative analysis of a-SMA. (C)Representative immunofluorescence image of a-SMA and vimentin, green, a-SMA; red,vimentin; blue, nuclei. Scale bars represent 20 μm. (D) The quantification of number of a-SMA and vimentin positivestaining cells per mm2. *p < 0.05 versus MI or Ad-GFP+MI,**p < 0.01 versus sham (n = 6).