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. 2017 May 24;7:2370. doi: 10.1038/s41598-017-02641-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA-binding activity in SPO-11. (a) The indicated concentration of SPO-11 was incubated with a 100 bp dsDNA. In lane 8, the reaction mixture was treated with proteinase K (PK) and SDS to release the DNA from the nucleoprotein complex. (b) The interaction of indicated concentration of SPO-11 with an immobilized 100 bp dsDNA was analyzed by surface plasmon resonance. (c) DNA binding activity of SPO-11 is temperature sensitive.