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. 2017 May 24;7:2344. doi: 10.1038/s41598-017-02454-0

Figure 2.

Figure 2

Lifetime measurements for H-M and H-H complexes; (a) Schematic representation of TAPIN experimental set up. (b) A typical fluorescence time trajectory. (c) and (d) indicate typical lifetime histograms for H-M (n = 297, blue) and H-H complexes (n = 274, dark grey), respectively. The insets show zoomed histograms in the range of 0–2000 ms. The histograms were fitted with the Lognormal function (red line). (e) The average lifetime for the H-M and H-H complexes, extracted from three independent experiments at pH 7.0 and room temperature (mean ± S.D.); the asterisks indicate statistical significance between the two groups using the Kolmogorov-Smirnov test (***p < 0.005). n is the number of data points.