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. 2017 May 24;7:2343. doi: 10.1038/s41598-017-02422-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-9 expression of different T cell subsets in infected hepatic lymphocytes. Female C57BL/6 mice were infected with 40 ± 5 S. japonicum cercariae per mouse. These mice were sacrificed at 5–6 weeks post infection. Single hepatic lymphocyte suspensions were stimulated for 5 hours with PMA and ionomycin supplemented with BFA. Cells were stained with different fluorophore-conjugated antibodies for CD3, CD4, CD8, γδTCR, NK1.1 and IL-9 for FACS analysis. Numbers in quadrants are percentages of cells in each expression phenotype. A representative of three independent experiments is shown.