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. 2017 May 24;7:2314. doi: 10.1038/s41598-017-01027-5

Figure 5.

Figure 5

miRNA-29c downregulates TTP by interacting with its 3′-UTR. (A) A predicted miRNA-29c target site resides at nucleotides 498–504 (shown in the white box) of the mouse TTP 3′-UTR and is highly conserved in several species. (B) The wild-type and mutant sequences of TTP 3′-UTR. (C) Differentiated podocytes were co-transfected with Wt or Mut TTP 3′-UTR-pmirGLO and miRNA-29c mimics or miR-control. After 36 h, cells were lysed and luciferase activities were detected by Dual-Luciferase Reporter Assay Kit. Luciferase activities were normalized to Renil-laluciferase. Results were obtained from three independent experiments. Data are shown as mean ± SEM. *P < 0.05 compared with Mut TTP 3′-UTR and miRNA-29c mimics co-transfected group.