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. 2017 May 24;7:2352. doi: 10.1038/s41598-017-02598-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Replication kinetics of the duck 3286/H7N9 variants on A549 cells. A549 cells were infected with the indicated viruses at an MOI of 0.01 and cultured at 37 °C in the presence of TPCK-trypsin at 0.2 ug/ml respectively. Culture supernatants were harvested at the indicated times and virus titers were determined by TCID₅₀ assay. Results are the average of three independent experiments. The viral titers in PB2 mutants were compared to the recombinant wild type virus using the two tailed, non-paired t-test. *p < 0.05.