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. 2017 May 24;7:2352. doi: 10.1038/s41598-017-02598-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(cont) Cytokine responses in the lungs of mice infected with duck 3286/H7N9 and its PB2 mutants. Cytokine levels (A) RANTES (B) MCP-3 (C) IFN-α (D) GM-CSF E) MIP-1β from virus infected lungs (n = 6 mice per virus group, days 3, 6 post-inoculation) were measured individually by the FlowCytomix system. Results from each time point are expressed as mean ± SD of six infected mice from two independent experiments. *The values were statistically compared with the wild type 3286/H7N9 using the two tailed, non-paired t-test. p < 0.05.