Figure 2.

Selective inhibition of DP-rings appears to be specific to PI4K inhibitors. (A) Synchronized rings treated at concentrations corresponding to the IC₉₀ of TQ (0.7 µM), ATQ (0.003 µM), LUM (0.06 µM), KDU691 (0.7 µM) and untreated control for six or 24 hours. Growth was monitored by HCI. (B) Dormancy was induced by exposing synchronized rings to 700 nM DHA for 6 hours. After washing and a further 18 hours of culture, the DP-rings were treated with the same panel of drugs and concentrations as for panel A for 24 hours. Parasite growth was monitored for seven days by HCI. For the DP-rings, growth in the presence of drugs (applied during the period of 24 to 48 hours) was normalized to the growth of parasites not exposed to drugs during the same period. Percent growth was measured by HCI using MitoTracker® Orange relative to DMSO. Data are from three biological experiments with technical duplicates (mean ± SEM % growth).