Table 3.
Sequencea | λAbs,max [nm]b | λEm,max [nm]b | ΦF b,c | τ1(α1) [ns]b,d | τ2(α2) [ns]b,d | <τ>[ns]e | kr [107 s−1]f | knr [108 s−1]g |
---|---|---|---|---|---|---|---|---|
GG | 373 | 453 | 0.22 | 4.9 (0.87) | 1.9 (0.13) | 4.5 | 4.9 | 1.7 |
UG | 368 | 452 | 0.20 | 4.1 (0.88) | 2.1 (0.12) | 3.8 | 5.3 | 2.1 |
GU | 373 | 460 | 0.22 | 4.8 (0.88) | 2.2 (0.12) | 4.5 | 4.9 | 1.7 |
AA | 371 | 457 | 0.23 | 4.6 (0.89) | 2.1 (0.11) | 4.3 | 5.3 | 1.8 |
AU | 370 | 459 | 0.23 | 4.4 (0.90) | 2.0 (0.10) | 4.2 | 5.5 | 1.8 |
CC | 371 | 452 | 0.25 | 4.9 (0.91) | 2.1 (0.09) | 4.7 | 5.3 | 1.6 |
GUUU | 370 | 456 | 0.22 | 4.5 (0.87) | 2.2 (0.13) | 4.2 | 5.2 | 1.9 |
GAUU | 373 | 459 | 0.22 | 4.6 (0.88) | 2.2 (0.12) | 4.3 | 5.1 | 1.8 |
UUUG | 371 | 456 | 0.23 | 4.3 (0.89) | 1.9 (0.11) | 4.1 | 5.6 | 1.9 |
aSequences are named after the bases flanking tCO (see Fig. 1b). Full sequences can be found in Supplementary Table S1. bMeasurements were performed in PBS buffer (100 mM Na+, pH 7.5). cFluorescence quantum yields are measured relative to the quantum yield of the potassium salt of the tCO-monomer in water (ΦF = 0.30)31. dThe amplitudes are indicated in parenthesis. eMean fluorescence lifetimes < τ > = ∑αiτi/∑αi. fRadiative rate constant, kr = ΦF/<τ>. gNon-radiative rate constant, knr = kr/ΦF − kr.