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. 2017 May 24;7:2346. doi: 10.1038/s41598-017-02498-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thrombolysis study with plasmin, urokinase and tissue plasminogen activator (t-PA). Thrombolysis profiles measured with the Halo assay method with 0.5 U/mL, 0.1 U/mL and 0.01 U/mL plasmin (A), with 400 U/mL, 200 U/mL and 100 U/mL urokinase (D) and with 0.35 nM, 0.7 nM, 1.4 nM, 3.5 nM and 7 nM t-PA (G). Activation times (At, black spots) and time to 50% lysis (T0.5, grey spots) (B,E and H) and maximal clot lysis rates (C,F and I) were presented for each concentration as mean values ± SD (n = 5, ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).