Fig. 2.

a ADCC activity differs for mAb1 despite being produced in the same cell type. Three different batches of proposed biosimilar mAb1 were produced by process 1 (red and green) or process 2 (orange) and tested for ADCC activity. Neither process condition was able to produce an antibody with ADCC activity similar to that of the originator mAb1 (blue). In contrast, b demonstrates similar ADCC activity for mAb2 despite being expressed in differing cell systems. The graph compares US-sourced (black) reference product and EU-sourced (blue) reference product produced in murine cells, compared with the mAb2 proposed biosimilar (red) produced in CHO cells. Ab antibody, ADCC antibody-dependent cellular toxicity, CHO Chinese hamster ovary, mAb monoclonal antibody