Table 1.
Sequences of siRNA, shRNA, miRNA and PCR primer.
| Name | Sequence (5’ → 3’) | Target position |
|---|---|---|
| CMPK-siRNA | ||
| Sense | GAAAGAUUGUACCAGUUGAtt | nt 424–442 |
| Antisense | UCAACUGGUACAAUCUUUCtt | |
| NC-siRNA | ||
| Sense | UUCUCCGAACGUGUCACGUTT | Scramble |
| Antisense | ACGUGACACGUUCGGAGAATT | |
| CMPK-shRNA | ||
| Sense | gatccGAAAGATTGTACCAGTTGAttcaagagaTCAACTGGTACAATCTTTCttttttg | nt 424–442 |
| Antisense | aattcaaaaaaGAAAGATTGTACCAGTTGAtctcttgaaTCAACTGGTACAATCTTTCg | |
| miR-130b-3p | ||
| miR-130b mimic | CAGUGCAAUGAUGAAAGGGCAU | nt 1824–1834 |
| miR-Ctrl | UCACAACCUCCUAGAAAGAGUAGA | |
| anti-miR-130b | AUGCCCUUUCAUCAUUGCACUG | nt 1824–1834 |
| anti-Ctrl | UCUACUCUUUCUAGGAGGUUGUGA | |
| CMPK PCR primer | ||
| Forward | TGTCAGCTCCCTCAGCGTC | nt 114–132 |
| Reverse | CGAGGACGAACACGACCAG | nt 255–273 |
| β-actin PCR primer | ||
| Forward | CATTGCCGACAGGATGCAG | nt 1008–1026 |
| Reverse | CTCGTCATACTCCTGCTTGCTG | nt 1155–1176 |
| miR-130b-3p primer | ||
| Forward | GGCAGTGCAATGATGAAAGG | |
| Reverse | GTGCAGGGTCCGAGGT | |
| U6 primer | ||
| Forward | CTCGCTTCGGCAGCACA | |
| Reverse | AACGCTTCACGAATTTGCGT | |
| miR-130b RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACatgccc | |
The target position in CMPK mRNA sequence (GenBank Accession: NM_016308) and β-actin mRNA sequence (GenBank Accession: NM_001101) is shown. Sequence in lowercase indicates a linker. NC, negative control; nt, nucleotide; PCR, polymerase chain reaction; siRNA, small interfering RNA; shRNA, short hairpin RNA.