Table 3.

Significant Associations of Short-Term Diesel Exhaust Exposure and CpG Site Methylation Difference (Postexposure − Preexposure) in Adults with Asthma (n = 16)

Chr	GWIS Locus	Probe	Probe Position (Build 37)	CpG Site Location	ΔFA* (Post − Pre)	ΔDE† (Post − Pre)	DE P Value	DE Adjusted P Value‡
5	ADCY2	cg04119977	7,826,972	ADCY2 (Body)	−0.015	−0.019	0.0011	0.041
5	ADCY2	cg10995381	7,877,198	MTRR (Body)	−0.013	−0.032	0.0011	0.041
11	DLG2	cg26449294	83,169,193	DLG2 (3′UTR)	−0.010	−0.021	4.64 × 10−5	0.017
11	DLG2	cg09080874	83,284,905	DLG2 (Body)	−0.015	−0.027	3.01 × 10−4	0.029
11	DLG2	cg27373604	83,372,714	DLG2 (5′UTR;Body)	−0.021	−0.026	0.0021	0.041
11	DLG2	cg08432013	83,393,570	DLG2 (Body;TSS200)	−0.010	−0.025	5.85 × 10−4	0.031
11	DLG2	cg02675969	83,526,604	DLG2 (Body)	−0.010	−0.017	0.0018	0.041
11	DLG2	cg05405389	84,386,472	DLG2 (Body)	−0.017	−0.035	0.0016	0.041
11	DLG2	cg18023263	84,403,466	DLG2 (Body)	−0.020	−0.022	0.0013	0.041
11	DLG2	cg14716968	84,635,906	DLG2 (TSS1500;Body)	−0.003	−0.037	4.28 × 10−4	0.029
11	DLG2	cg20275558	85,338,473	TMEM126B; DLG2 (TSS1500;TSS200)	0.011	0.042	1.07 × 10−4	0.020
11	DLG2	cg06698742	85,359,218	TMEM126A (5′UTR)	0.0009	0.0041	0.0014	0.041
18	MOCOS	cg19453250	33,710,783	SLC39A6; ELP2 (TSS1500;Body)	0.0076	0.024	0.0019	0.041

Definition of abbreviations: ADCY2 = adenylate cyclase 2; Chr = chromosome; DE = diesel exhaust exposure; DLG2 = discs, large homolog 2; ELP2 = elongator acetyltransferase complex subunit 2; FA = filtered air; GWIS = genome-wide interaction study; MOCOS = molybdenum cofactor sulfurase; MTRR = 5-methyltetrahydrofolate-homocysteine methyltransferase reductase; SLC39A6 = solute carrier family 39 member A6; TMEM126A = transmembrane protein 126A; TMEM126B = transmembrane protein 126B; UTR = untranslated region.

P values in bold have a value ≤0.05.

^*ΔFA: relative methylation change postexposure versus preexposure of filtered air.

^†ΔDE: relative methylation change postexposure versus preexposure of diesel exhaust.

^‡Adjusted P values using the false discovery rate method for multiple testing at a 5% level.