Figure 1.

Infectivity of the Fiber-Modified Adenoviral Vectors in Both Human and Murine Glioma Cell Lines

(A and B) Cells were infected with Ad5PK7-CMVGFP, Ad5RGD-CMVGFP, or Ad5-CMVGFP at an MOI of 300 VP/cell for 2 hr in (A) human glioma cell lines (U87 and U25) and non-neoplastic cells (normal human astrocytes and neuronal stem cells) and (B) a murine glioma cell line (GL261-OVA). The virus-containing medium was then removed and replaced with fresh medium containing 2% FBS. After 72-hr incubation, flow cytometric analysis was performed. Each data point and column is the average of three independent replicates. The mean ± SE is plotted. ***p < 0.001. NHA, normal human astrocyte; ns, not significant; NSC, neuronal stem cell; VP, viral particle.