Figure 5.

Increase of Antigen-Specific CD8+ within the Tumor Sites of Mice Treated with Adenoviral Vector

Five days after GL261-OVA implantation, mice were injected intracranially (i.c.) with PBS, replication-incompetent Ad5RGD-CMV-GFP, or replication-competent Ad5RGD-CMV-Δ24. Flow cytometric analyses of the brain of treated mice were performed 5 days after viral administration. (A and B) Both GFP and Delta-24 viruses recruit more tetramer-positive CD8 T cells and total CD8 T cells to the tumor, respectively. (C) The percentage of CD8+ T cells expressing the T cell receptor (TCR) for OVA in class I as determined by tetramer staining analyses is shown. Error bars are calculated as the mean ± SEM, with n = 5 per group. Significance was calculated using a one-way ANOVA followed by a Tukey’s post hoc test to compare individual groups. *p < 0.05.