Figure 5.

Decreased Expression of miR-1290 Increased the Sensitivity to 5-FU in SW480-5Fu_res and HCT116-5Fu_res Cells by Targeting hMSH2, Resulting in Altered Expression of Apoptosis-Related Proteins

(A and C) Representative flow cytometry analysis by using PE Annexin V and 7-Amino-Actinomycin (7-AAD) double staining in SW480-5Fu_res and HCT116-5Fu_res cells, which were transfected with miR-1290 inhibitor (anti-miR-1290), negative control (anti-NC), or miR-1290 plus si-hMSH2 and treated with 10 μM 5-FU or the same volume of DMSO as a control and incubated for 48 hr. Histograms represent the proportion of apoptotic cells presented as the mean ± SD from three independent experiments (**p < 0.01). (B and D) Representative western blots using the indicated antibodies in SW480-5Fu_res and HCT116-5Fu_res cells transfected and treated as described above.