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Frequency of ancestral rpoS819 (stationary sigma factor) and new rpoS819 allele variant (rpoS819_92) fluctuates and exhibits different patterns across replicates (rpoS:+46 and rpoS:+92). Other genes such as cpdA (a cyclic AMP diphosphoesterase) and rpoC, rpoB, and rpoA (RNA polymerase subunits) repeatedly appeared in multiple replicates.
