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Competitive binding of monoubiquitin and SUMO2 to Usp251–146 detected by 1H-15N HSQC titration experiments. CSPs are presented for the representative residues (A) G29 and T55 in UBA, (B) A111 in UIM1, and (C) S136 in UIM2 of the 15N-labeled Usp25 UBR with the sequential addition of monoubiquitin and SUMO2. To see this figure in color, go online.
