Figure 10.

Hop1CTD, but not full-length Hop1 or HORMA, exhibits a high affinity binding to the i-motif formed by meiosis-specific DSB from chromosome IV of S. cerevisiae. Reaction mixtures (20 μL) containing 0.5 nM ³²P-labeled C-rich ssDNA (A–C) or i-motif mutant DNA (D–F) or i-motif DNA (G–I) were incubated in the absence (lane 1) or the presence of (lanes 2–11) 150, 300, 450, 600, 750, 900, 1050, 1200, 1350, and 1500 nM of full-length Hop1, HORMA, or Hop1CTD, respectively. The solid triangle on top of the gel denotes increasing concentrations of the specified protein. (J) Given here is a graphical representation of the extent of protein binding to C-rich ssDNA, i-motif mutant, or i-motif structures. The extent of the formation of the protein–DNA complex in (A)–(I) is plotted versus varying concentrations of specified protein. Error bars indicate mean ± SE.