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. 2017 May 23;112(10):2056–2074. doi: 10.1016/j.bpj.2017.04.028

Figure 7.

Figure 7

The G-rich or C-rich sequences associated with a meiosis-specific DSB on chromosome IV of S. cerevisiae fold into G-quadruplex and i-motif structures, respectively. The S. cerevisiae strain W303 was transformed individually with a centromeric pRS416 plasmid expressing GFP or GFP tagged to a G-rich sequence (G plasmid) or GFP tagged to a C-rich sequence (C plasmid) or GFP tagged to their corresponding mutant sequences (G mutant or C mutant plasmid). Fluorescence and bright-field images were acquired with a confocal laser scan microscope. The panels show bright-field images (A)–(E), GFP fluorescence images (F)–(J), and merged fluorescence-bright field images (K)–(O) of the constructs above.