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The formation of G-quadruplex and i-motif structures in vivo lead to decreased GFP expression. (A) Given here is a schematic representation of the experimental design. (B) Histograms depict the coefficient of variation in the amount of GFP expression in S. cerevisiae cells containing the indicated recombinant constructs. Exponentially growing cells of S. cerevisiae strain W303 were serially diluted (OD600 = 0.2, 0.4, and 0.6) and used to measure GFP fluorescence.
