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. 2016 Apr 26;22(6):874–883. doi: 10.1038/mp.2016.59

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Functional screen of AD susceptibility loci in Drosophila. (a) Quantification of the eye size of flies co-expressing Tau and AD candidate gene loss-/gain-of-function constructs. Each symbol in the graph represents the median of the measurement of the size of 10 fly eyes per construct and control (30 values for the 3 pooled controls). Blue circles, squares and triangles represent RNAi, mutant and overexpression constructs, respectively (), and black circles represent controls (○). For RNAi, we plotted values normalized according to their origin (see Materials and Methods section). When the size of the eye was statistically different from the control (Wilcoxon test, Bonferroni correction, for RNAi with and without correction), we used red color (). Positive hits were re-tested and plotted with filled symbols (). The two horizontal lines mark the eye size range of the Tau expressing control. Constructs that are above the controls are Tau toxicity suppressors and the ones that are lower are enhancers. Vertical dashed and solid lines separate Drosophila genes and loci. The name of the Drosophila gene targeted by the construct is indicated below the two graphs, as well as the name of their human orthologs and the name of the locus they belong to. Positive hits are shaded in gray. (b) Representative images of the positive hits (scale bar 0.1 mm). Images for the Fak gene are available in Figure 2.

Inline graphic — Functional screen of AD susceptibility loci in Drosophila. (a) Quantification of the eye size of flies co-expressing Tau and AD candidate gene loss-/gain-of-function constructs. Each symbol in the graph represents the median of the measurement of the size of 10 fly eyes per construct and control (30 values for the 3 pooled controls). Blue circles, squares and triangles represent RNAi, mutant and overexpression constructs, respectively (), and black circles represent controls (○). For RNAi, we plotted values normalized according to their origin (see Materials and Methods section). When the size of the eye was statistically different from the control (Wilcoxon test, Bonferroni correction, for RNAi with and without correction), we used red color (). Positive hits were re-tested and plotted with filled symbols (). The two horizontal lines mark the eye size range of the Tau expressing control. Constructs that are above the controls are Tau toxicity suppressors and the ones that are lower are enhancers. Vertical dashed and solid lines separate Drosophila genes and loci. The name of the Drosophila gene targeted by the construct is indicated below the two graphs, as well as the name of their human orthologs and the name of the locus they belong to. Positive hits are shaded in gray. (b) Representative images of the positive hits (scale bar 0.1 mm). Images for the Fak gene are available in Figure 2.