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. 2017 Mar 27;6(6):602–610. doi: 10.1016/j.molmet.2017.03.009

Figure 1.

Figure 1

Decreased expression of β-klotho in white adipose tissue correlates with elevated levels of circulating FGF21. (A) Plasma FGF21 protein levels in male WT C57Bl/6 mice ad libitum fed chow or 60% high fat diet (HFD) for 16 weeks (n = 6–7/group). (B–G) Quantitative real-time PCR (QPCR) analysis of Klb and Fgfr1c mRNA expression in epididymal white adipose tissue (eWAT; B, C), liver (D–E), and hypothalamus (F–G) (n = 6–7/group). (H) Western blot analysis of KLB protein expression in eWAT of male WT C57Bl/6 mice ad libitum fed chow or 60% high fat diet (HFD) for 16 weeks. (I) Representative western blots of KLB protein expression in eWAT, brown adipose tissue (BAT), and liver of WT C57Bl/6 mice ad libitum fed 60% high fat diet (HFD) for the indicated time (5–6 mice/group). (J) Plasma FGF21 and insulin levels from the mice in (I). (K) Liver Fgf21 mRNA levels from mice in (I). Values are mean +/− SEM. (*, P < 0.05; **, P < 0.01; ***, P < 0.005; and #, P < 0.001 compared to WT; for (J) significance is relative to time 0).