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. 2017 Mar 27;6(6):602–610. doi: 10.1016/j.molmet.2017.03.009

Figure 2.

Figure 2

Generation of adipose-specific β-klotho transgenic mice. (A) Schematic of inducible β-klotho transgenic construct (inKLB). (B) Western blot analysis of KLB protein expression in epididymal white adipose tissue (eWAT) of 16 week chow-fed and high fat diet (HFD)-fed wild-type (WT) mice compared to HFD fed WT and adipose-specific β-klotho transgenic (KLB AdipoTG) mice. QPCR analysis of Klb and Tdtomato mRNA expression in eWAT (C, D), inguinal white adipose tissue (iWAT; E, F), brown adipose tissue (BAT; G, H), and liver (I, J) of WT and KLB AdipoTG mice fed chow or 60% HFD for 12 weeks (n = 7–11/group). (K) Western blot analysis of cytoplasmic and membrane KLB protein expression in epididymal white adipose tissue of wild-type (WT) and KLB adipose-specific transgenic mice (KLB AdipoTG). Values are mean +/− SEM. (*, P < 0.05; ***, P < 0.005; and #, P < 0.001 compared to WT; nd = not detected).