Figure 4.
White adipose tissue and systemic FGF21 sensitivity is not improved by preservation of β-klotho expression. (A) Plasma FGF21 and (B) hepatic Fgf21 mRNA levels in WT and KLB AdipoTG mice fed chow or HFD for 14 weeks (n = 8–9/group). (C) Western blot analysis of phospho-ERK1/2, total ERK1/2, phospho-FRS2α, phospho-MEK, and total MEK from eWAT of chow and HFD fed WT and KLB AdipoTG mice administered either vehicle or FGF21 (0.1 mg/kg) for 15 min. Values are mean +/− SEM. (#, P < 0.001 compared to WT).