Figure 3.

Impaired energy balance in adult onset deleted NPY-neuron specific IR-deficient mice. (A) Body weight curve of IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,tm mice measured on a weekly basis starting from the time of tamoxifen injection. (B) Body weights were measured after 10 weeks post-tamoxifen injection. (C–E) Body composition (lean and fat mass) determined by DXA scan and normalized to body weight in IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,m mice. (F–H) Bone mineral content (BMC), bone area, and bone mineral density (BMD) determined in IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,tm mice. (I) Absolute weights of adipose tissues from inguinal (WATi), epididymal (WATe), mesenteric (WATm), and retroperitoneal (WATr) depots and intrascapular brown adipose tissue (BAT) measured from IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,tm mice. (J) Basal daily food intake assessed in IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,tm mice. (K–M) Light and dark cycle energy expenditure (normalized to total lean mass), ambulatory activity, and respiratory exchange ratio determined in metabolic chambers in IR^lox/lox,tm and IR^lox/lox;NPY^Cre/+,tm mice. Data are means ± SEM of 7–10 mice per group. *p < 0.05, **p < 0.01.