TABLE 2.
Comparison of in vitro activities of posaconazole, voriconazole, and itraconazole tested against Aspergillus species by SYO and CLSI methodsa
| Species (no. of isolates tested)b | Test method | MIC (μg/ml) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PSC |
VRC |
ITC |
||||||||
| Range | Mode(s) | % EA | Range | Mode(s) | % EA | Range | Mode(s) | % EA | ||
| A. fumigatus (21) | SYO | 0.06 to 1 | 1 | 100 | 0.125 to >8 | 0.25 | 100 | 0.125 to >8 | 0.25 | 95.2 |
| CLSI | ≤0.03 to 2 | 1 | 0.06 to >8 | 0.125 | 0.25 to >8 | 1 | ||||
| A. flavus (19) | SYO | 0.06 to 0.125 | 0.125 | 100 | 0.125 to 1 | 0.25 | 100 | 0.03 to 0.25 | 0.06 | 94.7 |
| CLSI | ≤0.03 to 0.25 | 0.125 | 0.06 to 0.25 | 0.125 | 0.06 to 0.5 | 0.125 | ||||
| A. terreus (12) | SYO | 0.06 to 0.125 | 0.06 | 100 | 0.06 to 0.25 | 0.125 | 100 | 0.06 to 0.25 | 0.125 | 91.7 |
| CLSI | ≤0.03 to 0.25 | 0.25 | 0.06 to 0.125 | 0.06 | 0.125 to 0.5 | 0.25 | ||||
| A. niger (7) | SYO | ≤0.03 to 0.25 | 0.25 | 85.7 | 0.25 to 0.5 | 0.25 | 100 | 0.125 to 0.5 | 0.25 | 100 |
| CLSI | 0.06 to 0.25 | 0.25 | 0.06 to 0.25 | 0.25 | 0.5 to 1 | 0.5 | ||||
| A. tubingensis (6) | SYO | 0.125 to 0.25 | 0.125 | 100 | 0.25 to 0.5 | 0.5 | 100 | 0.25 to 0.5 | 0.25, 0.5 | 100 |
| CLSI | 0.125 to 0.5 | 0.125, 0.25, 0.5 | 0.125 to 0.5 | 0.25 | 0.5 to 2 | 0.5 | ||||
| A. nidulans (5) | SYO | 0.06 to 0.125 | 0.06 | 100 | 0.125 to 0.25 | 0.25 | 100 | 0.125 to 0.25 | 0.125 | 100 |
| CLSI | ≤0.03 to 0.06 | 0.03 | 0.06 to 0.25 | 0.06, 0.125 | 0.25 to 1 | 0.5 | ||||
| A. oryzae (5) | SYO | 0.125 to 0.5 | 0.125, 0.25 | 80.0 | 0.5 to >8 | 0.5, 1 | 100 | 0.125 to 0.5 | 0.125 | 100 |
| CLSI | ≤0.03 to 1 | 0.03 | 0.125 to 2 | 0.125 | 0.25 to 1 | 0.25 | ||||
| A. lentulus (3) | SYO | 0.03 to 0.125 | 0.03 | 100 | 2 to 4 | 2 | 100 | 0.06 to 0.125 | 0.125 | 100 |
| CLSI | ≤0.03 to 0.06 | 0.03 | 0.5 to 2 | ND | 0.25 to 0.5 | 0.25 | ||||
| A. (Neosartorya) species (3)c | SYO | 0.125 to 0.25 | 0.125 | 66.7 | 0.5 to 2 | ND | 100 | 0.25 to 0.5 | 0.25 | 100 |
| CLSI | ≤0.03 to 0.125 | 0.125 | 0.5 to 1 | 1 | 0.5 to 1 | 0.5 | ||||
| A. foetidus (3) | SYO | 0.125 to 0.25 | 0.125 | 100 | 0.5 to 1 | 0.5 | 100 | 0.5 | 0.5 | 100 |
| CLSI | ≤0.03 to 0.25 | ND | 0.25 to 0.5 | 0.25 | 0.25 to 2 | ND | ||||
| A. awamori (2) | SYO | 0.06 | 0.06 | 100 | 0.25 | 0.25 | 100 | 0.125 to 0.25 | ND | 100 |
| CLSI | ≤0.03 to 0.125 | ND | 0.125 to 0.25 | ND | 0.25 to 0.5 | ND | ||||
Posaconazole (PSC), voriconazole (VRC), and itraconazole (ITC) MICs were defined as the antifungal concentrations at which complete (100%) inhibition of growth of the Aspergillus species isolates was observed and are reported as the range and mode(s) (i.e., most frequent MIC[s] for each species). ND, not determined. For each species, the essential agreement (EA) between MIC values (± 2 2-fold dilutions) was calculated by comparison of MIC results obtained with the SYO method to those obtained with the CLSI method.
Except for A. nidulans, all of the less common or cryptic Aspergillus species listed belonged to the following Aspergillus sections, per molecular and/or proteomic-based identification: Fumigati (A. lentulus and Aspergillus [Neosartorya] spp.), Flavi (A. oryzae), and Nigri (A. tubingensis, A. foetidus, and A. awamori).
Data include 1 isolate each of A. (Neosartorya) hiratsukae, A. thermomutatus (Neosartorya pseudofischeri), and A. (Neosartorya) udagawae. In accordance with the recent taxonomists' recommendations for species for which a single-name nomenclature (i.e., keeping the name Aspergillus for all species of this genus) must be applied (17), the old teleomorphic name is indicated in parentheses.