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. 2017 May 24;61(6):e02193-16. doi: 10.1128/AAC.02193-16

TABLE 1.

Inhibition parameters of PER-2 and derived Arg220 and Thr237 mutants

PER-2	Clavulanic acid				Tazobactam
PER-2	k_inact (s⁻¹)	K_i (μM)	k_inact/K_i (μM⁻¹ s⁻¹)	Relative k_inact/K_i (%)^a	k_inact (s⁻¹)	K_i (μM)	k_inact/K_i (μM⁻¹ s⁻¹)	Relative k_inact/K_i (%)^a
Wild type	0.031 ± 0.001	0.064 ± 0.007	0.48 ± 0.07	100	0.047 ± 0.002	0.18 ± 0.02	0.26 ± 0.04	100
Mutant
Arg220Leu	0.045 ± 0.001	4.1 ± 0.2	0.0109 ± 0.0008	2.3	0.016 ± 0.001	10 ± 1	0.0016 ± 0.0004	0.6
Arg220Ser	0.055 ± 0.002	1.8 ± 0.1	0.031 ± 0.003	6.5	0.0172 ± 0.0005	2.8 ± 0.2	0.0062 ± 0.0007	2.4
Arg220Gly	0.11 ± 0.01	80 ± 10	0.0014 ± 0.0003	0.3	0.0157 ± 0.0006	31 ± 3	0.00050 ± 0.00007	0.2
Arg220His	0.032 ± 0.002	3.3 ± 0.5	0.010 ± 0.002	2.0	0.0150 ± 0.0005	5.1 ± 0.6	0.0029 ± 0.0004	1.1
Arg220Thr	ND^b	ND	0.0068 ± 0.0002	1.4	0.022 ± 0.001	11 ± 1	0.0019 ± 0.0004	0.7
Arg220Cys	0.31 ± 0.04	19 ± 3	0.016 ± 0.005	3.3	0.0187 ± 0.0004	6.0 ± 0.4	0.0031 ± 0.0002	1.2
Thr237Ala	0.027 ± 0.001	0.070 ± 0.009	0.38 ± 0.07	79.8	0.022 ± 0.002	0.27 ± 0.06	0.08 ± 0.02	30.6

^a

Relative to wild-type PER-2.

^b

ND, not determined.