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Melt curve comparisons between known and unknown sequence types. (A) HRM profiles of 4 clinical isolates (solid line, 1 ST131 isolate; dashed lines, 3 isolates of unknown sequence type) from the adk, gyrB, mdh, and recA multiplexed reaction mixture. (B) The four individual melt profiles from panel A are presented on the same graph. By running a known ST131 sample as a control, non-ST131 isolates can be visually identified by their lack of similarity to the ST131 melt profile.
