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. 2017 May 24;61(6):e00265-17. doi: 10.1128/AAC.00265-17

TABLE 1.

Cross-platform sensitivities and specificitiesa

Platform No. of isolates with indicated result determined by derivative melt curveb (no. determined by PCAc)
Sensitivity (%) of:
Specificity (%) of:
True positive False positive True negative False negative HRM PCA HRM PCA
Rotor-Gene Q 66 (66) 1 (3) 124 (122) 0 (0) 100 100 99.2 96.8
ABI 7500 Fast 66 2 123 0 100 98.4
a

Blind validation results of the HRM methodology evaluation of the panel of 191 clinical isolates, consisting of 66 ST131 and 125 non-ST131 E. coli isolates.

b

Determined by comparing the derivative melt curve to an ST131 standard.

c

Determined by ScreenClust HRM software principal component analysis and clustering.