TABLE 1.
Platform | No. of isolates with indicated result determined by derivative melt curveb (no. determined by PCAc) |
Sensitivity (%) of: |
Specificity (%) of: |
|||||
---|---|---|---|---|---|---|---|---|
True positive | False positive | True negative | False negative | HRM | PCA | HRM | PCA | |
Rotor-Gene Q | 66 (66) | 1 (3) | 124 (122) | 0 (0) | 100 | 100 | 99.2 | 96.8 |
ABI 7500 Fast | 66 | 2 | 123 | 0 | 100 | 98.4 |
Blind validation results of the HRM methodology evaluation of the panel of 191 clinical isolates, consisting of 66 ST131 and 125 non-ST131 E. coli isolates.
Determined by comparing the derivative melt curve to an ST131 standard.
Determined by ScreenClust HRM software principal component analysis and clustering.