TABLE 2.
Comparison of methodologies
| Procedure | Outline | Cost for analysisa | Time for results | Result |
|---|---|---|---|---|
| MLST | PCR, column purification, sample dilution, offsite sequencing | $78.96b | 2 to 3 days | Sequence type classification of all isolates |
| HRM screen | Sample dilution, HRM reaction | $4.28 to $5.18c | ∼3 h | Identification of ST131 E. coli |
a
Cost for analysis at time of article submission.
b
Price cited includes the cost of PCR reagents, column purification, and Sanger sequencing for each of the 7 MLST genes.
c
Price cited is the cost of the Qiagen DNeasy blood and tissue kit and HRM master mix from Qiagen or Thermo Fisher Scientific for both master mixes.