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The Acanthamoeba Neff strain separated into subpopulations after 72 h, as follows: P1, total cell population, analyzed by the flow cytometer; P2, cysts; P3, trophozoites, analyzed according to size and complexity of cells; P4, cysts; P5, precysts, analyzed by fluorescence emitted by the Congo red staining. (A) Control. (B) Cells after treatment with caffeine IC90. (C) Cells after treatment with maslinic acid IC90.
