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(a) Inhibitory effects of AC and AB compounds on in vitro pppGpp synthesis at a 100 μM concentration. Each experiment was performed in three biological replicates. Densitometric analysis was performed, and the values obtained were normalized with respect to the wild type (WT or 4). A Student t test was carried out to confirm the significance. The P value was <0.05 in the case of 6 and 7. (b) Dose-dependent inhibition of in vitro pppGpp synthesis ranging from 1 to 250 μM.
